JAC Advance Access published online on October 29, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg476
© 2003 by The British Society for Antimicrobial Chemotherapy
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Original article
1 Department of Infection,
Chungshan Medical University Hospital;
* Corresponding author. E-mail: mcyin{at}csmu.edu.tw.
Received 31 March 2003
; revised 16 September 2003
; accepted 26 August 2003
Objectives: The inhibitory effect
of garlic extract, diallyl sulphide and diallyl disulphide against
methicillin-resistant Staphylococcus aureus (MRSA)
infection in BALB/cA mice was studied. The influence of these agents
upon the levels of fibronectin, interleukin-6 and lipid oxidation
in MRSA-infected mice was examined. Methods: Garlic extract at 100% and
50%; diallyl sulphide (DAS) at 10% and 5%;
diallyl disulphide (DADS) at 1% and 0.5% were
used in this study. Sixteen clinical MRSA isolates obtained from
infected patients were used in this study (n = 16).
Mice were infected by injecting 200 µL
MRSA-PBS solution, which contained 107 cfu,
via the tail vein. At 16 h post-infection (p.i.), garlic extract,
DAS or DADS at 200 µL was administrated orally.
At 24 h p.i., mice were killed and blood, liver, kidney and spleen
of each mouse were collected. Plasma and the filtrate from each
organ and serial dilutions were used to determine colony count.
Plasma fibronectin level was determined by rabbit anti-rat fibronectin
antibody and quantified by ELISA. Interleukin-6 levels were determined
by commercial kit. Lipid oxidation was determined by measuring malondialdehyde
levels. Results: The oral administration of these agents
significantly decreased the viability of MRSA, in plasma, liver,
kidney and spleen (P < 0.05). MRSA infection
significantly increased fibronectin and interleukin-6 levels in
plasma of MRSA-infected mice (P < 0.05);
however, the oral administration of garlic extract and two diallyl
sulphides significantly reduced both fibronectin and interleukin-6
levels (P < 0.05). MRSA infection also
significantly enhanced lipid oxidation in plasma and three organs
(P < 0.05). The treatments of garlic extract
and two diallyl sulphides significantly decreased the malondialdehyde
level and showed antioxidant protection (P < 0.05). Conclusions: These data strongly supported the
conclusion that garlic extract, diallyl sulphide and diallyl disulphide
possessed multiple protective functions against MRSA infection,
in which diallyl sulphide and diallyl disulphide could be considered
as novel therapeutic agents for the treatment of MRSA infection.
Keywords: survival rate, fibronectin, interleukin-6, lipid
oxidation
Garlic extract and two diallyl sulphides inhibit
methicillin-resistant Staphylococcus aureus infection
in BALB/cA mice
2 Institute of Nutritional Science, Chungshan Medical
University, No. 110, Sec. 1, Chien Kuo N. Rd., Taichung City, Taiwan,
ROC
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