Journal of Antimicrobial Chemotherapy, Vol 40, 171-178, Copyright © 1997 by The British Society for Antimicrobial Chemotherapy
TR Walsh, RA Stunt, JA Nabi, AP MacGowan and PM Bennett
Clinical and environmental isolates of Aeromonas spp. (20 Aeromonas caviae,
33 Aeromonas veronii and 21 Aeromonas hydrophila) were examined for their
ability to yield mutants derepressed for beta-lactamase production and for
the distribution of the three chromosomally encoded beta-lactamase genes,
ampS and cepS from A. veronii bv. sobria and cphA from A. hydrophila. Of
these isolates, 100% and 96% of the isolates from A. hydrophila and A.
caviae, respectively, yielded beta-lactamase derepressed mutants but only
38% of A. veronii isolates yielded the resistant phenotype at 37 degrees C.
However, when tested at 30 degrees C, all isolates gave rise to the
derepressed mutants, indicating a temperature effect on the control
mechanism. All mutants had significantly higher beta-lactamase activity
against ampicillin, oxacillin, cephaloridine and imipenem. Hybridization
studies with cloned aeromonas beta-lactamase genes indicated that the
cephalosporinase gene, cepS, is almost ubiquitous for the three species
tested. The cphA gene cross-hybridized with all isolates of A. veronii and
A. hydrophila but not to A. caviae isolates. In contrast, hybridization
studies using ampS revealed that only 25% of A. caviae, 45% of A. veronii
and 38% of A. hydrophila tested carried the ampS gene or one closely
homologous to it. Nonetheless, strains that failed to hybridize with ampS
showed two serine beta-lactamases when analysed by isoelectric focusing.
ORIGINAL ARTICLES
Distribution and expression of beta-lactamase genes among Aeromonas spp
Bristol Centre for Antimicrobial Research and Evaluation, Department of Microbiology and Pathology, Medical School, University of Bristol, UK.
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